Retrospective biodosimetry using translocation frequency in a stable cell of occupationally exposed to ionizing radiation
نویسندگان
چکیده
Two cases of hematological malignancies were reported in an industrial radiography company over a year, which were reasonably suspected of being consequences of prolonged exposure to ionizing radiation because of the higher incidence than expected in the general population. We analyzed chromosomal aberrations in the peripheral blood lymphocytes from the other workers who had been working under similar circumstances as the patients in the company. Among the subjects tested, 10 workers who belonged to the highest band were followed up periodically for 1.5 years since the first analysis. The aim of this study was to clarify pertinence of translocation analysis to an industrial set-up where chronic exposure was commonly expected. To be a useful tool for a retrospective biodosimetry, the aberrations need to be persistent for a decade or longer. Therefore we calculated the decline rates and half-lives of frequency for both a reciprocal translocation and a dicentric chromosome and compared them. In this study, while the frequency of reciprocal translocations was maintained at the initial level, dicentric chromosomes were decreased to 46.9% (31.0-76.5) of the initial frequency over the follow-up period. Our results support the long-term stability of reciprocal translocation through the cell cycle and validate the usefulness of translocation analysis as a retrospective biodosimetry for cases of occupational exposure.
منابع مشابه
Retrospective assessment of radiation dose by Fluorescence In Situ Hybridization and evaluation of stable chromosomal aberrations
Estimation of absorbed dose for radiation workers or person involved in different radiological accident is the aim of biodosimetry. Cytogenetic methods are the most current and applicable biodosimetry tools. In chronic or protracted exposure, fluorescence in situ hybridization (FISH), stable chromosomal aberration is used for estimation of absorbed dose. For precise estimation of absorbed dose,...
متن کاملStable and unstable chromosome aberrations measured after occupational exposure to ionizing radiation and ultrasound.
AIM To evaluate chromosome aberration and fluorescent in situ hybridization (FISH) assays as a method to estimate of health risk, we monitored 9 male subjects occupationally exposed to low doses of both ionizing radiation and ultrasound during a period of over 3 years. METHODS Sampling was performed at 6-month intervals during a three-year period. First we used conventional chromosomal aberra...
متن کاملA Novel Biological Dosimetry Method for Monitoring Occupational Radiation Exposure in Diagnostic and Therapeutic Wards: From Radiation Dosimetry to Biological Effects
Background and Objective: Professional radiation workers are occupationally exposed to long-term low levels of ionizing radiation. Occupational health hazards from radiation exposure, in a large occupational segment of the population, are of special concern. Biological dosimetry can be performed in addition to physical dosimetry with the aim of individual dose assessment and biological effects....
متن کاملValidation of in vivo translocation frequency obtained by FISH with conventional G-banding Evaluation of ionizing radiation exposure for biodosimetry: Validation of Whole Chromosome
متن کامل
Variation of annual effective dose from external ionizing radiation among radiation workers of Bahawalpur Institute of Nuclear Medicine and Oncology (BINO), Pakistan
Background: The aim of the study was to analyze the effective dose record of occupationally exposed radiation workers at Bahawalpur Institute of Nuclear Medicine and Oncology department. Materials and Methods: Annual effective doses of occupationally exposed workers were measured by film badge dosimetry by sending the dosimeters to Radiation Dosimetry Laboratory (RDL), Pakistan Institute of Nuc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 56 شماره
صفحات -
تاریخ انتشار 2015